626 1717 1719 1720
2013/4/5
[edit]
| lane | clone ID | host | induction | purification | MW |
| 1 | 626,pT7A3-Taq_d289 | Nico21DE3plysN | 0.2 mM IPTG 25 oC o/n | Ni beads | 61KD/5.97 |
| 2 | 1717, pT7A3-6H-(TEV)-MBP-(3C) | Nico21DE3plysN | 0.2 mM IPTG 25 oC o/n | Ni beads | 49KD/5.46 |
| 3 | 1719, pT7A3-MBP-(E3C)-NRF2 | BL21DE3pRare2pLysS | 0.2 mM IPTG 25 oC o/n | Ni beads | 112KD/4.82 |
| 4 | 1720, pT7A3-MBP-(E3C)-Keap1 | BL21DE3pRare2pLysS | 0.2 mM IPTG 25 oC o/n | Ni beads | 115KD/5.57 |
| 5 | 1719, pT7A3-MBP-(E3C)-NRF2, fraction 38 | BL21DE3pRare2pLysS | 0.2 mM IPTG 25 oC o/n | 5ml HisTrap HP | 112KD/4.82 |
| 6 | 1719, pT7A3-MBP-(E3C)-NRF2, fraction 23 | BL21DE3pRare2pLysS | 0.2 mM IPTG 25 oC o/n | 5ml HisTrap HP | 112KD/4.82 |
| 7 | Cre, 3.9ug/ul | __ | __ | __ | __ |
| 8 | cre,4/2/2013 | __ | __ | __ | __ |
| 9 | 1624,pCold-6His-GST-(E)-(3C) | Nico21DE3plysN | 0.2 mM IPTG 25 oC o/n | Ni beads | 33KD/6.0 |