562 pEC-EcoR1

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Cloning of 562 pEC-EcoR1

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Purpose: Cloning of 562 pEC-EcoR1

Date: 2013-4---

Notebook:0

Page:

Clone ID:562

Experimenters: Shanyi

Results

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cloning

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gene synthesis


join two fragments by PCR



digest with Nde1+Xho1



Colony Screening

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Date: 2013-4-

Make miniprep and digest with Nde1+Xho1



cloning

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FIX ONE GAP,USE 562-6 as template,use EcoRI-1-1and EcoRI-1-6, EcoRI-1-7 and EcoRI-2-12 as primers

join two fragments by PCR


digest with Nde1+Xho1




Colony Screening

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Date: 2013-4-

Make miniprep and digest with Nde1+Xho1




Result intepretation/Summary

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Conclusion

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