1724-pT7A3-6His-sc-VVCap
Cloning of 1724-pT7A3-6His-sc-VVCap
[edit]Purpose: Cloning of 1724-pT7A3-6His-sc-VVCap
Date: 2013-5-8--2013-5-10
Notebook:0
Page:
Clone ID:1724
Experimenters: yingying
Results
[edit]cloning
[edit]
Colony Screening
[edit]Date: 2013-5-10
Make miniprep and digest with Nde1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]protein screening
[edit]2013-5-21
[edit]
Ni beads
| lane |
clone ID |
host |
induction |
purification |
MW |
| 1 |
1694,pT7A3-wtSM2 |
BL21(DE3)pRare2pLysS |
0.2 mM IPTG 25 oC o/n |
CBD beads, wrong buffer,need to do again |
33KD |
| 2 |
1724,pT7A3-6His-sc-VVCap |
NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
70KD |
| 3 |
1729,pCold-TF-(E3C)-3C |
BL21(DE3)pRare2pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
51KD+21KD |
| 4 |
1731,pT7A3-TurboEnterokinase |
BL21(DE3)pRare2pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
27KD |
| 5 |
1741,pIC-sfGFP |
NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
no his tag,supernatant run gel |
31KD |
| 6 |
1742,p8H-ELK-IN |
NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
15KD |
| 7 |
1743,pIC |
NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
4KD |
| 8 |
1744,p8HIC |
NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
5KD |
| 9 |
1745,p8HIC-sfGFP |
NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
15KD |
| 10 |
1748,pCold-8His-MBP-NRF2-6His |
BL21(DE3)pRare2pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads | |
| 11 |
1749,p7H-ELK-IN |
NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
15KD |