1735 pT7A30-Exo-Taq-sso7d
Cloning of 1735 pT7A30-Exo-Taq-sso7d
[edit]Purpose: Cloning of 1735 pT7A30-Exo-Taq-sso7d
Date: May , 2013
Notebook:
Page:
Clone ID: 1735
Experimenters: Shanyi
Results
[edit]Cloning
[edit]ligation using fragments from:
1248 pT7A3-DeepVent-Exo, Nde1 + Nco1 ==> exo
1249 pET-Taq_d289, Nco1 + Kpn1==> Taq
368 pET30b-sso7d-KX, Kpn1+ Xho1==> sso7d
to Nde1 + Xho1 digested 1727 pT7A30
Colony screen
[edit]Cloning
[edit]ligation using fragments from:
1248 pT7A3-DeepVent-Exo, Nde1 + Nco1 ==> exo
1249 pET-Taq_d289, Nco1 + Kpn1==> Taq
to Nde1 + Kpn1 digested 1734
Colony screen
[edit]DIGEST WITH NdeI+NcoI+KpnI
