1661 1674 1683
1661 1674 1683
[edit]Protein Expression
[edit]
2013/3/5
| Lane |
Name |
Clone ID |
Host |
Induction |
Purification |
| M |
Fermentas PageRuler Plus |
||||
| 1 |
pT7A3-MBP-(3c)-NRF2 |
1661 clone 1 |
4 ml, BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 2 |
pT7A3-MBP-(3c)-KEAP1 |
1661 Clone 2 |
4 ml, BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 3 |
pCold-GST-(3c)-NRF2 |
1674 |
4 ml, BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads (wrong, should use Glutathione agarose beads, no his tag) |
| 4 |
pCold-GST-(3c)-NRF2 |
1674 |
4 ml, BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads (wrong, should use Glutathione agarose beads, no his tag) |
| 5 |
pCold-MBP-(3C)-NRF2 |
1683 |
4 ml, BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 6 |
pCold-MBP-(3C)-NRF2 |
1683 |
4 ml,BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 7 |
pT7A3-MBP-(3c)-KEAP1 |
1625 |
4 ml,BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 8 |
pCold-MBP-(3C)-KEAP1 |
1676 |
4 ml,BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 9 | |||||
| 10 | Purified MBP |