1625 1626 1656
Protein Expression
[edit]2013/2/11
| Lane |
Name |
Clone ID |
Host |
Induction |
Purification |
| M |
Invitrogen SeeBlue plus2 |
||||
| 1 |
pT7A3-MBP-(3c)-KEAP1 |
1625 |
4 ml, BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 2 |
pT7A3-MBP-(3c)-KEAP1 |
1625 |
4 ml, BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 3 |
pCold-GST-(3c)-KEAP1 |
1656 |
4 ml, BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads (wrong, should use Glutathione agarose beads) |
| 4 |
pCold-GST-(3c)-KEAP1 |
1656 |
4 ml, BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads (wrong, should use Glutathione agarose beads) |
| 5 |
pET28-cold-NRF2 |
1626 |
4 ml, BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 6 |
pT7A3-MBP-i3C |
1554 |
4 ml,NiCO21(DE3), pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 7 |
pT7A3-6His-GST-i3C |
1601 |
4 ml,NiCO21(DE3), pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
| 8 |