1701-pMAL-c5E-KEAP1
Cloning of 1701-pMAL-c5E-KEAP1
[edit]Purpose: Cloning of 1701-pMAL-c5E-KEAP1
Date: 2013-3-8----
Notebook:0
Page:
Clone ID:1701
Experimenters: yingying
Results
[edit]subcloning
[edit]
Colony Screening
[edit]Date: 2013/3/9
Make miniprep and digest with Nde1 + HindIII
digest clone #4 with EcoR1
Transform clone Bl21 Star (DE3)/pRare2pLysS with #4, grow on LB/amp/chl plate
Result intepretation/Summary
[edit]Conclusion
[edit]Protein expression of KEAP1 clones
[edit]| clone ID |
expression |
ge image |
|
| 1625,pT7A3-MBP-(3c)-KEAP1 |
yes |
1625 1626 1656 |
|
| 1656,pCold-GST-(3c)-KEAP1 |
need to use GST beads |
1625 1626 1656 |
|
| 1676,pCold-MBP-(3C)-KEAP1 |
yes |
1661 1674 1683 |
|
| 1701,pMAL-c5E-KEAP1 |
yes |
||
| 1720,pT7A3-MBP-(E3C)-Keap1 |
no |
626 1717 1719 1720 |
|