1709-pLVX-CMV-iCre-IRES-mCherry
Cloning of 1709-pLVX-CMV-iCre-IRES-mCherry
[edit]Purpose: Cloning of 1709-pLVX-CMV-iCre-IRES-mCherry
Date: 2013-6-3---2013-6-5
Notebook:0
Page:
Clone ID:1709
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using
iCre-Xho1-R
TGA TCG CTC GAG TCA GTC CCC ATC CTC CAG CAG CCT CAC, 3_11_2013
iCre-BamH1-F
TAG ACA GGA TCC GCC GCC ACC ATG GTG C, 2_12_2013
digest with BamH1 + Xho1
vector:174 pLVXPlus2-IRES-mCherry
Colony Screening
[edit]Date: 2013-6-5
Make miniprep and digest with BamH1 + Xho1