1751-pBad24-SOC- HOC
Cloning of 1751-pBad24-SOC- HOC
[edit]Purpose: Cloning of 1751-pBad24-SOC- HOC
Date: 2013-6-11---2013-6-13
Notebook:0
Page:
Clone ID:1751
Experimenters: yingying
Results
[edit]cloning
[edit]
insert: 1766 pUC57-SOC-HOC digest with EcoR1+Hind111+Bsa1 at 37C
band 4: vector: 546 pBad24-sfGFP digest with EcoR1+Hind111
Colony Screening
[edit]Date: 2013-6-13
Make miniprep and digest with EcoR1+Hind111
digest with Xba1+Hind111
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1751-pBad24-SOC- HOC
[edit]Purpose: Cloning of 1751-pBad24-SOC- HOC
Date: 2013-6-12---2013-6-13
Notebook:0
Page:
Clone ID:1751
Experimenters: yingying
Results
[edit]cloning
[edit]
vector: 546 pBad24-sfGFP digest with EcoR1+Hind111
1766 pUC57-SOC-HOC digest with EcoR1+Hind111, cut the band and gel extraction ,then digest with Bsa1 again at 50C
run gel
cut large band and small band seperately, and do ligation with vector seperately
Colony Screening
[edit]Date: 2013-6-13
Make miniprep and digest with EcoR1+Hind111
large band ligation digestion result:
large band ligation digestion result: EcoR1+Hind111 , Xba1+Hind111
small band ligation digestion result:
Result intepretation/Summary
[edit]Conclusion
[edit]