1757-pT7A3-MBP-(3C)-FN3K
Cloning of 1757-pT7A3-MBP-(3C)-FN3K
[edit]Purpose: Cloning of 1757-pT7A3-MBP-(3C)-FN3K
Date: 2013-6-6---2013-6-7
Notebook:0
Page:
Clone ID:1757
Experimenters: yingying
Results
[edit]cloning
[edit]
subclone from
Harvard Kinase Plate HRG007900, 79
Well: G10
3,donor vector: pDNR-DUAL
4,acceptor vector: 1565 pT7A3-MBP-(3C)-sfGFP2
Enzyme: Sal1 + HindIII
Colony Screening
[edit]Date: 2013-6-7
Make miniprep and digest with Sal1 + HindIII
2013-6-12
652 pT7A30-hIFNA2 and 1757pT7A3-MBP-(3C)-FN3K digest with Sal1+Hind111
1757pT7A3-MBP-(3C)-FN3K digest with AlwN1
Sequence result show: this is PCK1 Plate: HRG007900, well: G02 ,
should clone again
Result intepretation/Summary
[edit]Conclusion
[edit]Cloning of 1757-pT7A3-MBP-(3C)-FN3K
[edit]Purpose: Cloning of 1757-pT7A3-MBP-(3C)-FN3K
Date: 2013-6-13---2013-6-17
Notebook:0
Page:
Clone ID:1757
Experimenters: yingying
Results
[edit]cloning
[edit]
subclone from
Harvard Kinase Plate HRG007900, 79
Well: G10
I,donor vector: pDNR-DUAL
V,acceptor vector: 1565 pT7A3-MBP-(3C)-sfGFP2
Enzyme: Sal1 + HindIII
Colony Screening
[edit]Date: 2013-6-17
Make miniprep and digest with Sal1 + HindIII/AlwN1
