556-pET28b-Sma1

Cloning of 556-pET28b-Sma1

Purpose: Cloning of 556-pET28b-Sma1

Date: 2013-6-3---2013-6-7

Notebook:0

Page:

Clone ID:556

Experimenters: yingying

Results

cloning

Colony Screening

Date: 2013-6-7

Make miniprep and digest with:nde+xho1

fix of Cloning of 556-pET28b-Sma1

Purpose: fix of Cloning of 556-pET28b-Sma1

Date: 2013-6-20---2013-6-24

Notebook:0

Page:

Clone ID:556

Experimenters: yingying

Results

cloning

556-4 above as template

1-1 to 1-17

1-16 to 1-20

template:pcr1+pcr2

primer :1-1 and  1-20

lane 2: vector 1225pET28b-T7-Endo1

Colony Screening

Date: 2013-6-24

Make miniprep and digest with:nde+xho1