635 636 638 640 1756 1759 1762 1763 1753

Protein Expression

2013/6/24
SHANYI

Lane	Name	Protein Name	Clone ID	Host	Induction	Purification	pI/Mw	Expression
1	pT7A3-Not1	Not1	635	5 ml, Nico21(DE3)plysN	0.2 mM IPTGRT o/n	Ni beads	5.27 / 44045.61	yes
2	pT7A3-Xba1	Xba1	636	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.58 / 25010.26	?
3	pT7A3-Nco1	Nco1	638	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.14 / 27156.67	?
4	pT7A3-EcoR1	EcoR1	640	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.52 / 32913.20	?
5	pCold-6H-(TEV)-MBP-(3C)	6H-(TEV)-MBP-(3C)	1756	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	4.88 / 47543.17	yes
6	pCold-6H-(TEV)-MBP-(3C)-Keap1	6H-(TEV)-MBP-(3C)-Keap1	1759	5 ml, BL21DE3pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	5.64 / 115376.57	yes
7	pCold-6H-(TEV)-MBP-(3C)-Keap1	6H-(TEV)-MBP-(3C)-Keap1	1759	5 ml, Nico21(DE3)pRare2pLysS	0.2 mM IPTG RT o/n	Ni beads	5.64 / 115376.57	yes
M	PageRuler Plus Prestained protein ladder
8	pT7A3--MBP-(3C)-Bst-Exo-	MBP-(3C)-Bst-Exo	1762	5 ml, Nico21(DE3)plysN	0.2 mM IPTG RT o/n	Ni beads	5.00 / 113222.51	yes
9	pT7A30-Tks-sso7d	Tks DNA Polymerase fused to sso7d	1763	5 ml, Nico21(DE3)plysN	0.2mM IPTG RT o/n	Ni beads	8.56 /98654.27	yes
10	pT7A3-ZB2-Keap1	ZB2-Keap1	1753	5 ml, Nico21(DE3)pRare2pLysS	0.2 mM IPTG RT o/n	SP Sepharose Fast Flow	6.35 / 76758.17	?