1661 1682 1683 1719 1748 1752 1771 1779

Date:2013-7-5

Lane	Name	Host	Induction	Purification	MW	Conclusion
1	1661,pT7A3-MBP-(3c)-NRF2	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	112KD	yes
2	1682, pCold-6His-GST-(E)-(3C)-NRF2	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	97KD	yes
3	1683,pCold-MBP-(3C)-NRF2	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	113KD	yes
4	1719,T7A3-MBP-(E3C)-NRF2	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	112KD	no
5	1748,pCold-8His-MBP-NRF2-6His	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	120KD	no
6	1752,pCold-TF-(E3C)-NRF2	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	120KD	yes
7	1771,pT7A3-MBP-(3C)-PCK1	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	110KD	yes
8	1779, pTCold-MBP-(3C-TEV)-NRF2	4 ml, NiCO21(DE3), pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	113KD	yes
9	1779, pTCold-MBP-(3C-TEV)-NRF2	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	113KD	yes