643 645 652 1721 1731 1736 1757 1764 1771

2013-7-9

Ni beads

lane	clone ID	Host	Induction	Purification	MW		Conclusion
1	643,pT7A3-Nhe1	4ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	38KD		yes
2	645,pT7A3-Pst1	4ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	37KD		weak
3	652, pT7A30-hIFNA2	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	20KD weak
4	1721, pT7A3-MBP-(E3C)-SOD	4ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	60KD		only MBP
5	1731,pT7A3-TurboEnterokinase	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	27KD		?
6	1736,pT7A3-His-NusA	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	56KD		yes
7	1757,pT7A3-MBP-(3C)-FN3K	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	81KD		weak
8	1764,pT7A3-T4-Pnk	4ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	37KD		yes
9	1771,pT7A3-MBP-(3C)-PCK1	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	120KD		yes