1764-pT7A3-T4-Pnk
Cloning of 1764-pT7A3-T4-Pnk
[edit]Purpose: Cloning of 1764-pT7A3-T4-Pnk
Date: 2013-4-1----2013-4-5
Notebook:0
Page:
Clone ID:1764
Experimenters: yingying
Results
[edit]cloning
[edit]
A : vector:PET28b
B: vector 1454 pEC
Colony Screening
[edit]Date: 2013-4
Make miniprep and digest with Nde1+xho1
Result intepretation/Summary
[edit]Conclusion
[edit]Purpose: Cloning of 1764-pT7A3-T4-Pnk
Date: 2013-6-3---2013-6-7
Notebook:0
Page:
Clone ID:1764
Experimenters: yingying
Results
[edit]cloning
[edit]
vector:1279-pT7A3
Colony Screening
[edit]Date: 2013-6-7
Make miniprep and digest Nde1+Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]protein screening
[edit]
Ni beads
| lane |
clone ID |
Host |
Induction |
Purification |
MW |
Conclusion | |
| 1 |
643,pT7A3-Nhe1 |
4ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
38KD |
yes | |
| 2 |
645,pT7A3-Pst1 |
4ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
37KD |
weak | |
| 3 |
652, pT7A30-hIFNA2 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
20KD weak |
||
| 4 |
1721, pT7A3-MBP-(E3C)-SOD |
4ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
60KD |
only MBP | |
| 5 |
1731,pT7A3-TurboEnterokinase |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
27KD |
? | |
| 6 |
1736,pT7A3-His-NusA |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
56KD |
yes | |
| 7 |
1757,pT7A3-MBP-(3C)-FN3K |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
81KD |
weak | |
| 8 |
1764,pT7A3-T4-Pnk |
4ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
37KD |
yes | |
| 9 |
1771,pT7A3-MBP-(3C)-PCK1 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
120KD |
yes | |