1774 pCMV5-hIgG-Lc-Keap1

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Cloning of 1774 pCMV5-hIgG-Lc-Keap1

Purpose: Cloning of 1774 pCMV5-hIgG-Lc-Keap1

Date: 2013-7--9-

Notebook:05846

Page:

Clone ID:1774

Experimenters: Shanyi

Results

cloning

PCR using 1623 pLenti6.2/V5-KEAP1 as template

digest PCR products with Nhe1 + Kpn1,

then ligate to Nhe1 + Kpn1 digested 1590 pCMV5-hIgG-Lc-TagGFP-Fc-no-intron-(3c)-22nt-IRES-TagRFP-WPRE-OriP

Colony Screening

Date: 2013-7-

Make miniprep and digest Nhe1 + Kpn1

Result intepretation/Summary

Conclusion

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Cloning