1739-pT7A3-ZB2-wtSM2
Cloning of 1739-pT7A3-ZB2-wtSM2
[edit]Purpose: Cloning of 1739-pT7A3-ZB2-wtSM2
Date: 2013-5-6---2013-5-9
Notebook:0
Page:
Clone ID:1739
Experimenters: yingying
Results
[edit]cloning
[edit]
Colony Screening
[edit]Date: 2013-5-9
Make miniprep and digest with Nde1 + Xho1
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1739-pT7A3-ZB2-wtSM2
[edit]Purpose: Cloning of 1739-pT7A3-ZB2-wtSM2
Date: 2013-7-15---2013-7-18
Notebook:0
Page:
Clone ID:1739
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR wtSM2 using
template is 1680pCold-GST-wtSM2
WtSM2-1
WtSM2-Xho1-R
| lane |
1 |
2 |
| PCR product |
1753,pT7A3-ZB2-Keap1 | |
| enzymes |
Xho1 + Nde1 | |
Colony Screening
[edit]Date: 2013-7-18
Make miniprep and digest with Nde1 + Xho1
clone5 for sequencing
Result intepretation/Summary
[edit]Conclusion
[edit]