1781-pTCold-MBP-(3C-TEV)-PKARIIb

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Cloning of 1781-pTCold-MBP-(3C-TEV)-PKARIIb

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Purpose: Cloning of 1781-pTCold-MBP-(3C-TEV)-PKARIIb

Date: 2013-7-17---2013-7-19

Notebook:0

Page:

Clone ID:1781

Experimenters: yingying

Results

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cloning

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PCR using 1728 as template
ratPKARIIb-Nhe1-F
PKARIIb-Xho1-R


lane
 3
 4

PCR product
 1677, pTCold-MBP-(3C)
enzymes
            Nhe1+Xho1


Colony Screening

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Date: 2013-7-19

Make miniprep and digest Nhe1+Xho1



Result intepretation/Summary

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Conclusion

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