1035-pET28b-Tth-Ligase-K294R
Cloning of 1035-pET28b-Tth-Ligase-K294R
[edit]Purpose: Cloning of 1035-pET28b-Tth-Ligase-K294R
Date: 2012-5-10----2012-5-17
Notebook:0
Page:
Clone ID:1035
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using
1stPCR:pET28b-Tth-Ligase 1022 as template, Tth-Lig-Nde1-F + TagLiase-K294R-R
2ndPCR: pET28b-Tth-Ligase 1022 as template, TagLiase-K294R-F + TagLiase-BamH1-R
3rdPCR 1stPCR+2ndPCR as template, Tth-Lig-Nde1-F +TagLiase-BamH1-R
| band 2 |
band 3 | |
| PCR product |
pET28b-Tth-Ligase 1022 | |
| enzymes |
Nde1 + BamH1 | |
Colony Screening
[edit]Date: 2012-5-17
Make miniprep and digest with Nde1 + BamH1
clone 8 may be right, need to sequence
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>
Cloning of 1035-pET28b-Tth-Ligase-K294R
[edit]Purpose: Cloning of 1035-pET28b-Tth-Ligase-K294R
Date: 2013-7-9---
Notebook:0
Page:
Clone ID:1035
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR using
1stPCR:pET28b-Tth-Ligase 1022 as template, Tth-Lig-Nde1-F + TagLiase-K294R-R
2ndPCR: pET28b-Tth-Ligase 1022 as template, TagLiase-K294R-F + TagLiase-BamH1-R
3rdPCR 1stPCR+2ndPCR as template, Tth-Lig-Nde1-F +TagLiase-BamH1-R
| band 2 |
band 3 | |
| PCR product |
pET28b-Tth-Ligase 1022 | |
| enzymes |
Nde1 + BamH1 | |
Colony Screening
[edit]Date: 2013-7-23
Make miniprep and digest with Nde1 + BamH1
