1758 1762 1779 1759
Protein Purification
[edit]2013/7/16
SHANYI
| Lane |
Name |
Protein Name |
Clone ID |
Host |
Purification |
pI/Mw |
Expression | |
| 1 |
pCMV5-hIgG-Lc-FN3K |
FN3K |
1758 |
293EF |
Histrap excel |
7.15 / 62756.42 |
no | |
| 2 |
||||||||
| 3 |
pCMV5-hIgG-Lc-PKARIIb |
PKARIIb |
1726 |
293EF |
Histrap excel |
5.52 / 75918.93 |
no | |
| 4 | ||||||||
| 5 |
PageRuler Plus Prestained protein ladder |
|
|
|||||
| 6 |
pTCold-MBP-(3C-TEV)-NRF2 |
NRF2 |
1779 |
Nico21(DE3)pRare2pLysS |
NRF20 0.36mg/ml |
Ni column Amylose column Histrap Amylose beads Flowthrough |
4.67 / 68345.09 |
|
| 7 |
pTCold-MBP-(3C-TEV)-NRF2 |
NRF2 |
1779 |
Nico21(DE3)pRare2pLysS |
NRF20
1mg/ml |
4.67 / 68345.09 |
||
| 8 |
pTCold-MBP-(3C-TEV)-NRF2 |
1779 |
Nico21(DE3)pRare2pLysS |
|
After TEVdigestion elute from Histrap |
|
||
| 9 |
pTCold-MBP-(3C-TEV)-NRF2 |
1779 |
Nico21(DE3)pRare2pLysS |
|
After TEVdigestion
elute from Amylose beads |
|
||
| 10 |
keapI |
|
||||||
| 11 |
keapI |
|
|