Agarose-Gel-Electrophoresis

1. Make 1% gel

1.6 g agarose (for electrophoresis).

160 ml 0.5 X TAE

boil in microwave until agarose beads are dissloved.

2. Cool to ~ 55 oC, add 5 ul 1000 X GRGreen in water

3. Pour to tray, wait for 15 to 30 min to solidify. You can also put the gel tray at 4 oC for faster cooling.

4. Replace buffer if you want to do cloning.

5. Run gel at 150 or 200 V, 15 to 30 min.

6. Take a gel picture. Remove gloves before touching the blue light box power switch and camera.

Note: Never touch camera and memory card using your gloves. $1000 deduction from your salary if you do.