1800-pCold-6His-TEV-MBP
Cloning of 1800-pCold-6His-TEV-MBP
[edit]Purpose: Cloning of 1800-pCold-6His-TEV-MBP
Date: 2013-7-22---2013-7-24
Notebook:0
Page:
Clone ID:1800
Experimenters: yingying
Results
[edit]cloning
[edit]
1. PCR TEV from 432 pMBP-7xHis-TEV
TEV-Kpn1-F
TEV-MBP-R
2,PCR MBP from 1281 pMAL-p5E as template
TEV-MBP-F
MBP-Sal1-stop-R2
3. Join two fragments using
TEV-Kpn1-F
MBP-Sal1-stop-R2
| lane |
3 |
4 |
| PCR product |
v19 pET28-cold | |
| enzymes |
Kpn1 + Sal1 |
Kpn1 + Xho1 |
Colony Screening
[edit]Date: 2013-7-24
Make miniprep and digest Kpn1+Bgl11
Result intepretation/Summary
[edit]Conclusion
[edit]
Cloning of 1800-pCold-6His-TEV-MBP
[edit]Purpose: Cloning of 1800-pCold-6His-TEV-MBP
Date: 2013-7-24--2013-7-26
Notebook:0
Page:
Clone ID:1800
Experimenters: yingying
Results
[edit]cloning
[edit]
1. PCR TEV from 432 pMBP-7xHis-TEV
TEV-Kpn1-F
TEV-MBP-R
2,PCR MBP from 1281 pMAL-p5E as template
TEV-MBP-F
MBP-Sal1-stop-R2
3. Join two fragments using
TEV-Kpn1-F
MBP-Sal1-stop-R2
| lane |
3 |
4 |
| PCR product |
v19 pET28-cold | |
| enzymes |
Kpn1 + Sal1 |
Kpn1 + Xho1 |
Colony Screening
[edit]Date: 2013-7-26
Make miniprep and digest Kpn1 + Bgl11
clone1,3,4 may be right ,need to sequencing
Result intepretation/Summary
[edit]Conclusion
[edit]