483-pET28b-TthPol

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Cloning of 483-pET28b-TthPol

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Purpose: Cloning of 483-pET28b-TthPol

Date: 2013-7-26---2013-7-31

Notebook:0

Page:

Clone ID:483

Experimenters: yingying

Results

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cloning

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PCR from ATCC Genomic DNA from Thermus thermophilus Strain DSM 7039 [HB27]
ATCC BAA-163D-5

TthPol-Nde1-F
TthPol-EcoR1-R


lane
 1
 2

 PCR product
 1029,pHisGST
enzymes
    EcoR1 + Nde1





Colony Screening

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Date: 2013-7-31

Make miniprep and digest with EcoR1 + Nde1




Result intepretation/Summary

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Conclusion

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