631 1497 1542 1719 1748 1765 1780 1781 1782 1797

2013-7-30

lane	clone ID	Host	Induction	Purification	MW(KD)	Conclusion
1	631,pT7A3-IL-22	4ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	17	NO
2	1497,pT7A3-ToughTaq	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 37 oC o/n	Ni beads	70	Weak
3	1542,pT7A3-sso7d-Native-ToughTaq	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 37 oC o/n	Ni beads	70	Weak
4	1719,pT7A3-MBP-(E3C)-NRF2	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	112	Yes
5	1748,pCold-8His-MBP-NRF2-6His	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	114	Yes
6	1765,pT7A3-T4DNAPol	4ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	105	Yes
7	1780,pT7A3-T7-RNAPol-Y639F	4ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	99	no
8	1781,pTCold-MBP-(3C-TEV)-PKARIIb	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	93	Yes
9	1782,pT7A3-Tth-Ligase	4ml,NiCO21(DE3)pLysN	0.2 mM IPTG 25 oC o/n	Ni beads	77	Yes
10	1797, pCold-6His-3C-MBP	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	62	MBP and 3C seperated