622 pT7A3-8H-IL-2

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Cloning of 622 pT7A3-8H-IL-2

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Purpose: Cloning of 622 pT7A3-8H-IL-2

Date: 2013-4---

Notebook:0

Page:

Clone ID:622

Experimenters: Shanyi

Results

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cloning

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gene synthesis


digest with Nde1+Xho1

Colony Screening

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Date: 2013-4-26

Make miniprep and digest with Nde1+Xho1


send 622-6 to sequence, IL-2 is right,but the vector is not pT7A3-8H,need to do subclone.

cloning

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digest with Nde1+Xho1



Colony Screening

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Date: 2013-4-26

Make miniprep and digest with Nde1+Xho1




Result intepretation/Summary

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Conclusion

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