1809-pCold-MBP-(E3C)-FN3K
Cloning of 1809-pCold-MBP-(E3C)-FN3K
[edit]Purpose: Cloning of 1809-pCold-MBP-(E3C)-FN3K
Date: 2013-8-2---2013-8-6
Notebook:0
Page:
Clone ID:1809
Experimenters: yingying
Results
[edit]cloning
[edit]PCR from DNA isolated from
Harvard Kinase Plate HRG007900, 79
Well: G10
FN3K-Nde1-F
FN3K-Xho1-R
| lane |
1 |
2 |
| PCR product |
1804, pCold-MBP-(E3C) | |
| enzymes |
Nde1 + Xho1 | |
Colony Screening
[edit]Date: 2013-8-6
Make miniprep and digest with Nde1 + Xho1/AlwN1
Result intepretation/Summary
[edit]Conclusion
[edit]