1807-pCold-MBP-(a3C)-MCS-3C

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Cloning of 1807-pCold-MBP-(a3C)-MCS-3C

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Purpose: Cloning of 1807-pCold-MBP-(a3C)-MCS-3C

Date: 2013-8-2---2013-8-7

Notebook:0

Page:

Clone ID:1807

Experimenters: yingying

Results

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cloning

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PCR 3C from1420 pDB.His.MBP.3C
1 ul of 10 uM SD-3C-F1
1 ul of 1 uM SD-3C-F2
3C-Sal1-R



lane
 1
 2

 PCR product
 1804 pCold-MBP-(E3C)
enzymes
 HindIII + Sal1
 HindIII + Xho1


Colony Screening

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Date: 2013-8-7

Make miniprep and digest with HindIII +Spe1




Result intepretation/Summary

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Conclusion

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