1219 pCDNA3.3-Fc(3C)
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Date: 2012-6-10
Notebook:
Page:
Clone ID: 1219
Experimenters: Paul
Cloning
[edit]6/10/2012.
PCR: ssFc-BamH1-F1 + 10% ss-Fc-F5 + Fc-HRV3C-Nhe1-Kpn1-R6, template is pFUSE-hIgG1-Fc2-IAB, 1101
KAPA, 20 cycles.
digest PCR product with BamH1+ Kpn1
digest pCDNA3.3, 1208 with BamH1+ Kpn1, ligate and transform Top10
Colony Screening by Restriction Digestion
[edit]
Digested lanes 1-6 with BAMHI and EcoRI and digested lanes 7-12 with Nhe1, positive for lanes samples 2, 4, 5 and 6.
Submitted sample 4 for sequencing.
2012-7-16
1219 digest detect
