1222-pCMV5-Fc(3C)-Dest
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Cloning of 1222-pCMV5-Fc(3C)-Dest
[edit]Purpose: Cloning of 1222-pCMV5-Fc(3C)-Dest
Date: 2012-7-10---2012-7-17
Notebook:0
Page:
Clone ID:1222
Experimenters: yingying
Results
[edit]subcloning
[edit]2012-7-9
| lane 1 |
lane 2 | |
| 448 pLex-CMV5-CuO |
1223 pCDNA3.3-Fc(3C)-Dest | |
| enzymes |
Nde1 + BamH1 | |
Colony Screening
[edit]Date: 2012-7-12
Make miniprep and digest with Nde1 + BamH1
clone 3,4,5,6,7,8 may be right
2012-7-17
clone 3 digest detect:
lane 1:Nde1+BamH1
lane 2:Nde1+Hind111
clone 3 right
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>