1324-pCMV5-Fc(3C)-VVDP-SVLT
Cloning of 1324-pCMV5-Fc(3C)-VVDP-SVLT
[edit]Purpose: Cloning of 1324-pCMV5-Fc(3C)-VVDP-SVLT
Date: 2012-8-14---2012-8-15
Notebook:0
Page:
Clone ID:1324
Experimenters: yingying
Results
[edit]cloning
[edit]
| lane 5 |
lane 6 | |
| PCR product |
1224 pCMV5-Fc(3C)-Dest-SVLT | |
| enzymes |
Nhe1 + Not1 | |
1224 vector should not have three lanes
Colony Screening
[edit]Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
<br>
<br>
<br>
Cloning of 1324-pCMV5-Fc(3C)-VVDP-SVLT
[edit]Purpose: Cloning of 1324-pCMV5-Fc(3C)-VVDP-SVLT
Date: 2012-8-30---2012-9-4
Notebook:0
Page:
Clone ID:1324
Experimenters: yingying
Results
[edit]cloning
[edit]
| lane 9 |
lane 10 | |
| PCR product |
1224 pCMV5-Fc(3C)-Dest-SVLT | |
| enzymes |
Nhe1 + Not1 | |
Colony Screening
[edit]Date: 2012-9-4
Make miniprep and digest with Nhe1 + Not1
not the right size
9/8/2012, digest miniprep with Nhe1 + Not1. no insert.
Result intepretation/Summary
[edit]- &lt;br&gt;
Conclusion
[edit]&lt;br&gt;
Cloning of pCMV5-Fc(3C)-VVDP-WPRE-OriP
[edit]Purpose: Cloning of pCMV5-Fc(3C)-VVDP-WPRE-OriP
Date: 2012-9-28 ~2012-10-5
Notebook:0
Page:
Clone ID:
Experimenters: Yingying
Results
[edit]PCR
[edit]2012-9-28
digest PCR product( lane3) with Nhe1+Not1
digest 1349pCMV5-Fc-(3c)-Dest-WPRE-oriP with Nhe1+Not1
Colony Screening
Date: 2012-10-5
mini prep and digest with Nhe1+Not1
clone 5 is right
Result intepretation/Summary
[edit]