1328 pCMV5-Fc(3C)-Dest
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Cloning of 1328_pCMV5-Fc(3C)-Dest
[edit]Purpose: Cloning of 1328_pCMV5-Fc(3C)-Dest
Date: 2012-8-10 to 2012-8-13
Notebook:
Page:
Clone ID: 1328
Experimenters: yingying
Results
[edit]cloning
[edit]Date:2012-2-10 8/10/2012
digest pCMV5-Fc(3C) 1262 with Nhe1 + Not1 --> vector
digest 1224 pCMV5-Fc(3C)-Dest-SVLT with Nhe1 + Not1 --> gateway cassette, truncated
ligate and transform ccdb-survial
Colony Screening
[edit]Date:2012-8-13
Make miniprep and digest with Nhe1 + Not1
all right
Gateway reaction
2012-8-15----2012-8-16
clony screening: digest with Nhe1 + Not1
clone 3,4 may be right;
7:control, 1328 plasmid