1344 pDB.His.MBP-(3C)-PLK1wt
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Cloning of 1344 pDB.His.MBP-(3C)-PLK1wt
[edit]Purpose: Cloning of 1344 pDB.His.MBP-(3C)-PLK1wt
Date: August 24, 2012
Notebook:
Page:
Clone ID: 1344
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 24, 2012
Cloning Method
PCR from 73 pLex-PLK1-wt
PLK1-Nhe1-F
cagtacGCTAGCATGAGTGCTGCAGTGACTGC
PLK1-EcoR1-R
(CAACCGTCTCAAGGCCTCCTAAgaattc ctagac)
gtctaggaattcTTAGGAGGCCTTGAGACGGTT
See lane 4
Digested DNA (see lane 4) with RE Nhe1 + EcoR1
Digested vector 1207 (see lane 3) with RE Nhe1 + EcoR1
Cut out bands, purify, ligate together, transform
Colony Screening
[edit]2012-8-27
Nhe1 and EcoR1
Result intepretation/Summary
[edit]
Conclusion
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