1353 pCMV5-Fc(3C)-RFP-WPRE
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Cloning of 1353 pCMV5-Fc(3C)-RFP-WPRE
[edit]Purpose: Cloning of 1353 pCMV5-Fc(3C)-RFP-WPRE
Date: August 21, 2012
Notebook:
Page:
Clone ID: 1353
Experimenters: Jennifer Truong
Results
[edit]Cloning
[edit]Date: August 21, 2012
Subclone RFP from 1305 pCMV5-Fc(3C)-TagRFP using Nhe1 + Not1
Digested insert 1305 pCMV5-Fc(3C)-TagRFP (lane 1) and vector 1336 (lane 2) with RE Nhe1 + Not1
Cut out bands, purify and ligate together, transform
Colony Screening
[edit]2012-8-27
Nhe1 + Not1
2012-9-14
Result intepretation/Summary
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Conclusion
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