1411-pCMV5-IgK-Flag-6His-Fc(3c)-Dest-WPRE-oriP

Cloning of 1411-pCMV5-IgK-Flag-6His-Fc(3c)-Dest-WPRE-oriP

Purpose: Cloning of 1411-pCMV5-IgK-Flag-6His-Fc(3c)-Dest-WPRE-oriP

Date: 2012-10-9---2012-10-12

Notebook:0

Page:

Clone ID:1411

Experimenters: yingying

Results

cloning

PCR Fc from 1361 pCDM8-like-nSEBOV-delta-thr-Fc

Fc-3C-Nhe1-R
1 ul of 10 uM Fc-3C-Nhe1-R
1 ul of 10 uM mIgK-BamH1-F1
1 ul of 1 uM mIgK-BamH1-F2
1 ul of 1 uM mIgK-BamH1-F3
1 ul of 1 uM mIgK-BamH1-F4

Digest PCR product (lane7) with BamH1 + Nhe1, ligate with BamH1 + Nhe1 digested vector 1349 pCMV5-Fc-(3c)-Dest-WPRE-oriP (lane8)

Colony Screening

Date: 2012-10-12

Make miniprep and digest with BamH1 + Nhe1

need to sequence

Result intepretation/Summary

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Conclusion

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