1426-pCMV5-TPA-Dest2-(3c)-Fc-WPRE-OriP
Cloning of 1426-pCMV5-TPA-Dest2-(3c)-Fc-WPRE-OriP
[edit]Purpose: Cloning of 1426-pCMV5-TPA-Dest2-(3c)-Fc-WPRE-OriP
Date: 2012-10-23---2012-10-25
Notebook:0
Page:
Clone ID:1426
Experimenters: yingying
Results
[edit]cloning
[edit]
PCR Gateway Cassette out from the new cassette 1336 pCMV5-Fc(3C)-Dest-WPRE
GW-Nhe1-F2 +GW-Not1-Kpn1-R
| lane3 |
lane4 | |
| PCR product |
1391,pCMV5-TPA-TagGFP-(3c)-Fc-WPRE-OriP | |
| enzymes |
Nhe1 + Kpn1 | |
Colony Screening
[edit]Date: 2012-10-25
Make miniprep and digest with Nhe1 + Kpn1
clone 1,2,3,4,6 need to sequence
Result intepretation/Summary
- <br>
Conclusion
[edit]<br>
<br>
<br>
<br>
Cloning of 1426-pCMV5-TPA-Dest2-(3c)-Fc-WPRE-OriP
[edit]Purpose: Cloning of 1426-pCMV5-TPA-Dest2-(3c)-Fc-WPRE-OriP
Date: 2012-11-26---2012-12-4
Notebook:0
Page:
Clone ID:1426
Experimenters: yingying
Results
[edit]cloning
[edit]PCR Gateway Cassette out from the new cassette 1336 pCMV5-Fc(3C)-Dest-WPRE
GW-Nhe1-F2 +GW-Not1-Kpn1-R
| lane3 |
lane4 | |
| PCR product |
1391,pCMV5-TPA-TagGFP-(3c)-Fc-WPRE-OriP | |
| enzymes |
Nhe1 + Kpn1 | |
Colony Screening
[edit]Date: 2012-11-30
PCR Screening: CMV5+GW-Not1-Kpn1-R
Grow 2,5,8,11
2012-12-3 mini prep and digest with Nhe1 + Kpn1
1,2,3,4 the same with (2,5,8,11 above),
need to sequencing
Result intepretation/Summary
[edit]- &lt;br&gt;
Conclusion
[edit]&lt;br&gt;
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<br>
<br>