1426-pCMV5-hIgG-LC-Dest2-(3c)-Fc-WPRE-OriP
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Cloning of 1426-pCMV5-hIgG-LC-Dest2-(3c)-Fc-WPRE-OriP
[edit]Purpose: Cloning of 1426-pCMV5-hIgG-LC-Dest2-(3c)-Fc-WPRE-OriP
Date: 2013-3
Notebook:0
Page:
Clone ID:1426
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR Gateway Cassette out from the new cassette 1336 pCMV5-Fc(3C)-Dest-WPRE
GW-Nhe1-F2 +GW-Not1-Kpn1-R
| lane5,6 |
lane7 | |
| PCR product |
1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP | |
| enzymes |
Nhe1 + Kpn1 | |
Colony Screening
[edit]Date: 2013
Make miniprep
Digest with Pst1 sould get 3 bands(5068bp, 1570bp, 3851bp) , Parental vector should get 1 band(1570bp)
Digest with Nhe1 + Kpn1
gateway393+1426
digest with psti
Result intepretation/Summary