143 pT7A3-MBP-(Tev)-GST-(3C)

redo

10/27/2018 Redo PCR 

need to check 143 clone #2.

sequencing: clone #2 no priming

11/5/2018, use new primers 143-F2
ATATATTCatg ACT AGT ggcaaaatcgaagaaggtaaac

143-MBP-R2         
GAACCGGTGCCTCCACCGCCCTGGAAG TAAAGGTTTTC

digest MBP-GST with BspH1-Nde1, clone into NcoI-Nde1 digested vector 1851

11/8/2018