1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP
Cloning of 1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP
[edit]Purpose: Cloning of 1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP
Date: 2012-12-15---2012-12-16
Notebook:0
Page:
Clone ID:1470
Experimenters: yingying
Results
[edit]cloning
[edit]
lane 3:digest PCR product with Not1 + BglIII,
lane 4:digest 1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP with BamH1 + Not1
wrong
Colony Screening
[edit]Date: 2012-
Make miniprep and digest with
Result intepretation/Summary
[edit]- <br>
Conclusion
[edit]<br>
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Cloning of 1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP
[edit]Purpose: Cloning of 1470-pCMV5-IgG-LC-Cripto-Fc(3c)-WPRE-OriP
Date: 2012-12-20---2012-12-27
Notebook:0
Page:
Clone ID:1470
Experimenters: yingying
Results
[edit]cloning
[edit]2012-12-20
Template: 599 pLX304-cripto
1 ul of 10 uM IgG-LC-BglII-BamH1-Nhe1-F1
1 ul of 1 uM IgG-LC-BglII-BamH1-Nhe1-F2
1 ul of 10 uM Cripto-Not1-R
lane 1(low lane):digest PCR product with Not1 + BglIII,
lane 2:digest 1395 pCMV5-hIgG-Lc-TagGFP-Fc(3c)-WPRE-OriP with BamH1 + Not1
Colony Screening
[edit]Date: 2012-12-27
Make miniprep and digest with BamH1 + Kpn1
Result intepretation/Summary
[edit]- &lt;br&gt;
Conclusion
[edit]&lt;br&gt;
&lt;br&gt;
<br>
<br>