1542 1544 1545 1627 1679 1680

2013/3/28

lane	name	clone ID	host	induction	purification	MW
1	pT7A3-sso7d-Native-ToughTaq	1542	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	62KD
2	pT7A3-VVCap-L	1544	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	34KD
3	pT7A3-VVCap	1545	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	34KD
4	pT7A3-SOD	1627	4 ml, BL21(DE3)Star, pRare2-pLysS	0.2 mM IPTG 25 oC o/n	Ni beads	16KD
5	pCold-MBP-SM2	1679	4 ml,NiCO21(DE3)pLysN,	0.2 mM IPTG 25 oC o/n	Ni beads	74KD
6	pCold-GST-wtSM2	1680	4 ml,NiCO21(DE3)pLysN,	0.2 mM IPTG 25 oC o/n	get lysis supernatant to run gel	94KD