1552-pT7A3-MBP-(3C)-T3-DNA-Ligase
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Cloning of 1552-pT7A3-MBP-(3C)-T3-DNA-Ligase
[edit]Purpose: Cloning of 1552-pT7A3-MBP-(3C)-T3-DNA-Ligase
Date: 2013-1-28---2013-1-30
Notebook:0
Page:
Clone ID:1552
Experimenters: Shanyi
Results
[edit]subcloning
[edit]subclone from 1518 pET28b-T3-DNA-Ligase to 1514 pT7A3-MBP-(3C) using Nde1 + Xho1
| band 1 |
band 2 | |
| 1514 pT7A3-MBP-(3C) |
1518 pET28b-T3-DNA-Ligase | |
| enzymes |
Nde1 + Xho1 | |
Colony Screening
[edit]Date: 2013-1-30
Make miniprep and digest with Nde1 + Xho1.
Result intepretation/Summary
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Conclusion
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