1588 pT7A3-MBP-(3c)-Exo-VII
Jump to navigation
Jump to search
Cloning of 1588 pT7A3-MBP-(3c)-Exo-VII
[edit]Purpose: Cloning of 1588 pT7A3-MBP-(3c)-Exo-VII
Date: 2013-3-7---
Notebook:0
Page:
Clone ID:1588
Experimenters: Shanyi
Results
[edit]PCR
[edit]PCR from genomic DNA isolated from Escherichia coli str. K-12 substr. MG1655
Cut out the band, digest PCR products and 1514 pT7A3-MBP-(3C) with Nde1 + Xho1
Colony Screening
[edit]Date: 2013-
Make miniprep and digest withNde1 + Xho1
send 1588-6 to sequence, missing 1 C
Cloning of 1588 pEC-Exo-VII
[edit]Purpose: Cloning of 1588 pEC-Exo-VII
Date: 2013-4----
Notebook:0
Page:
Clone ID:1588
Experimenters: Shanyi
Results
[edit]PCR
[edit]PCR from genomic DNA isolated from Escherichia coli str. K-12 substr. MG1655
Cut out the band, digest PCR products and 1454 with Nde1 + Xho1
Colony Screening
[edit]Date: 2013-
Make miniprep and digest withNde1 + Xho1
