1629 pCold-GST-(3C)

C onstruct  pCold-GST-(3C) vector 1629

Purpose: Cloning of 1629-pCold-GST-(3C)

Date: 2013-1-25---2013-1- 26

Notebook:0

Page:

Clone ID:1629

Experimenters: yingying

Results

PCR

pcr1

Make cold promoter by gene synthesis

pcr2
PCR GST from pDB.His.GST
Cold-GST-F+GST-3C-Nde1-R

pcr1+pcr2

digest pcr product  with BglII  + Nde1

Clone into v19 pET28-cold using BglII + Nde1

Colony Screening

Date: 2013-1-

Make miniprep and digest with

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C onstruct  pCold-GST-(3C) vector 1629

Purpose: Cloning of 1629-pCold-GST-(3C)

Date: 2013-2-1---2013-2-4

Notebook:0

Page:

Clone ID:1629

Experimenters: yingying

Results

PCR

pcr1

PCR from v19 pET28-cold using

Cold-BglII-F + Cold-GST-R

pcr2
PCR GST from pDB.His.GST
Cold-GST-F+GST-3C-Nde1-R

pcr1+pcr2

Cold-BglII-F+GST-3C-Nde1-R

Colony Screening

Date: 2013-2-4

Make miniprep and digest with BglII + Nde1

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