1659-pCold-MBP-(3C)-MCS
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Cloning of 1659-pCold-MBP-(3C)-MCS
[edit]Purpose: Cloning of 1659-pCold-MBP-(3C)-MCS
Date: 2013-2-20----2013-2-20
Notebook:0
Page:
Clone ID:1659
Experimenters: yingying
Results
[edit]cloning
[edit]
Digest 1611 pCold-MBP-(3C) with Nde1 + Xho1, gel purify
ligate to annealed oligos Nde1-MCS-Xho1-T + Nde1-MCS-Xho1-B.
Colony Screening
[edit]Date: 2013-2-22
Make miniprep and digest with Spe1 + BglII
Result intepretation/Summary
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Conclusion
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