165 pT7A3-MBP-(TEV)

Redo

Clone annealled oligos into Xba1-Nco1 digested  2369 pT7A3-lc-MBP-(TEV)
2094-T
CTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATAT
    TTTATTAAAACAAATTGAAATTCTTCCTCTATATAGTAC
2094-B
CATGATATATCTCCTTCTTAAAGTTAAACAAAATTATTT

2094  should not grow on Kan plate, but there are colonies. so it is contaminated clone, 

1/2/2019, digestion NcoI+ Xho1

http://www.excellgen.net/eln/images/8/8e/165_168_digestion.JPG