1666-pT7A3-GST-(E3C)

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Cloning of 1666-pT7A3-GST-(E3C)

Purpose: Cloning of 1666-pT7A3-GST-(E3C)

Date: 2013-2-22----2013-2-25

Notebook:0

Page:

Clone ID:1666

Experimenters: yingying

Results

cloning

digest vector 1665 pT7A3-GST-(TEV) with Nde1 + BamH1,
ligate to annealled oligos Nde1-3C-BamH1-T + Nde1-3C-BamH1-B

Colony Screening

Date: 2013-2-25

Make miniprep and digest with:Xba1 + Pst1

Result intepretation/Summary

Conclusion

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Cloning