1677-pTCold-MBP-(3C)
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Cloning of 1677-pTCold-MBP-(3C)
[edit]Purpose: Cloning of 1677-pTCold-MBP-(3C)
Date: 2013-3-4---2013-3-7
Notebook:0
Page:
Clone ID:1677
Experimenters: yingying
Results
[edit]cloning
[edit]
pcr2 :the same with 1611 pcr2
pcr1+pcr2
Colony Screening
[edit]Date: 2013-3-7
Make miniprep and digest with BglII + Nde1
Result intepretation/Summary
[edit]Conclusion
[edit]protein screening
[edit]
| lane |
name |
clone ID |
host |
induction |
purification |
MW | |
| 1 |
pT7A3-T5-Exo |
1567 |
4 ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
21KD/5.95 | |
| 2 |
pCold-TF-(3c) |
1614 |
4 ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
55.9KD/5.19 | |
| 3 |
pET28b-Nde1 |
1673 |
4 ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
44KD/8.41 | |
| 4 |
pTCold-MBP-(3C) |
1677 |
4 ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
44KD | |
| 5 |
pCold-C(3C)-MBP-6H |
1692 |
4 ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
46KD/5.77 | |
| 6 |
pT7A3-wtSM2 |
1694 |
4 ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads, should use CBD beads |
33KD/6.30 | |
| 7 |
pCold-MBP-(3C)-TEV |
1696 |
4 ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
72KD/6.04 | |
| 8 |
pCold-GST-(3c)-wt3C |
1697 |
4 ml,NiCO21(DE3)pLysN |
0.2 mM IPTG 25 oC o/n |
Ni beads |
21KD/8.47 | |
| 9 |
pT7A3-MBP-(E3C)-p53wt |
1707 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
88KD/5.76 | |
| 10 |
pET28b-hINFA2 |
1711 |
4 ml,
BL21(DE3)Star, pRare2-pLysS |
0.2 mM IPTG 25 oC o/n |
Ni beads |
21KD/5.95 | |