1768-pET28b-6H-(Tev)-Tat-HA

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Cloning of 1768-pET28b-6H-(Tev)-Tat-HA

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Purpose: Cloning of 1768-pET28b-6H-(Tev)-Tat-HA

Date: 2013-7-12---2013-7-17

Notebook:0

Page:

Clone ID:1768

Experimenters: yingying

Results

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cloning

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gene synthesis

Nco1-6H-Tev-Tat-HA-Nde1-1 to Nco1-6H-Tev-Tat-HA-Nde1-4



lane
 3
 4

 PCR product
 pET28b
enzymes
     Nco1 + Nde1


Colony Screening

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Date: 2013-7-17

Make miniprep and digest with Nco1 + Nde1



Result intepretation/Summary

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Conclusion

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