1771-pT7A3-MBP-(3C)-PCK1

Cloning of 1771-pT7A3-MBP-(3C)-FN3K

Purpose: Cloning of 1771-pT7A3-MBP-(3C)-FN3K

Date: 2013-6-6---2013-6-7

Notebook:0

Page:

Clone ID:1771

Experimenters: yingying

Results

cloning

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subclone from <br>Harvard Kinase Plate HRG007900, 79<br>Well: G02<br>3,donor vector: pDNR-DUAL<br>4,acceptor vector: 1565 pT7A3-MBP-(3C)-sfGFP2<br>Enzyme: Sal1 + HindIII

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Colony Screening

Date: 2013-6-7

Make miniprep and digest with Sal1 + HindIII

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2013-6-12<br>

652 pT7A30-hIFNA2 and 1757pT7A3-MBP-(3C)-FN3K digest with Sal1+Hind111<br>

1757pT7A3-MBP-(3C)-FN3K digest with AlwN1<br>

Sequence result show:&nbsp; this is PCK1&nbsp; Plate: HRG007900, well: G02 ,<br>

clone 1771

clone 1757 should subclone again