1776 pLVX-Flu A/WSN/33 M15/88E-TagRFP
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Cloning of1776 pLVX-Flu A/WSN/33 M15/88E-TagRFP
[edit]Purpose: Cloning of 1776 pLVX-Flu A/WSN/33 M15/88E-TagRFP
Date: 2013-7--2-
Notebook:05846
Page:
Clone ID:1776
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR use 1346 pET30b-A/WSN/33 M15/88E as template
digest PCR products with Xho1 + BamH1, then ligate to Xho1 + BamH1 digested 47pLVX-TagRFP
Colony Screening
[edit]Date: 2013-7-
Make miniprep and digest with Xho1 + BamH1
Result intepretation/Summary
[edit]Conclusion
[edit]Redo Cloning of1776 pLVX-Flu A/WSN/33 M15/88E-TagRFP
[edit]Purpose: Cloning of 1776 pLVX-Flu A/WSN/33 M15/88E-TagRFP
Date: 2013-7--9-
Notebook:05846
Page:
Clone ID:1776
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR use 1346 pET30b-A/WSN/33 M15/88E as template
digest PCR products with Xho1 + BamH1, then ligate to Xho1 + BamH1 digested 47pLVX-TagRFP
Colony Screening
[edit]Date: 2013-7-
Make miniprep and digest with Xho1 + BamH1
