1777 pLVX-Flu A/WSN/33 M15/88R-TagGFP
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Cloning of1777 pLVX-Flu A/WSN/33 M15/88R-TagGFP
[edit]Purpose: Cloning of 1777 pLVX-Flu A/WSN/33 M15/88R-TagGFP
Date: 2013-7--2-
Notebook:05846
Page:
'Clone ID:1777
Experimenters: Shanyi
Results
[edit]cloning
[edit]PCR use 1011 pET30b-FluASeg-7 as template
digest PCR products with Xho1 + BamH1, then ligate to Xho1 + BamH1 digested 46 pLVX-TagGFP-2
Colony Screening
[edit]Date: 2013-7-
Make miniprep and digest with Xho1 + BamH1